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笔者自80年起曾用小白鼠经台盼兰活体注射,碘苏木素中性红染色法染结缔组织撕片,以显示成纤维细胞、巨噬细胞、肥大细胞,胶原纤维及弹性纤维。但此法显示成纤维细胞形态大多数是显示其核,用于教学不够理想,现采用结缔组织撕片银染法染色,使成纤维细胞和肥大细胞形态均非常清晰,并易与巨噬细胞相区别。方法与步骤:1.取材取幼年健康小白鼠一只,从尾静脉注射用生理盐水配制的0.5%台盼兰溶液0.5 ml,隔天再注射一次,次日,用乙醚麻醉,取腹部皮下结缔组织一小块,取时应尽量少损伤血管,以免血液粘在皮下组织上。在载玻片上制成铺
The author has used mouse to trypan blue in vivo injection since 1980, and Iodine hematoxylin neutral red staining dye connective tissue to show fibroblasts, macrophages, mast cells, collagen fibers and elastic fibers. However, this method shows that the majority of fibroblast morphology shows its nucleus, which is not ideal for teaching. It is stained with silver staining of connective tissue to make the morphology of fibroblasts and mast cells very clear, and is easy to interact with macrophages Difference Methods and steps: 1. Take a young healthy young mice, injected 0.5% trypan blue solution 0.5% formulated with saline from the tail vein injection again the next day, the next day, anesthetized with ether, abdominal subcutaneous connective Tissue a small piece, when taken should minimize damage to the blood vessels, so as not to blood cling to the subcutaneous tissue. Made on a glass slide shop