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目的探讨去铁酮(defriprone,DFP)对铝染毒大鼠脑组织中甘氨酸(Gly)、γ-氨基丁酸(GABA)两种抑制性氨基酸类神经递质及铝和必需元素影响。方法将35只健康清洁级Wistar雄性大鼠按体重随机分为5组,分别为阴性对照组和铝单独染毒组以及铝+低、中、高剂量DFP组,每组7只,采用灌胃方式染毒,每天1次,每周连续染毒5 d,间隔2 d。前8周,阴性对照组给予生理盐水(1 ml/d),其他4组给予AlCl3溶液(354.7 mg/kg)。后2周,阴性对照组与铝染毒组给予生理盐水(1 ml/d);铝+DFP低、中、高剂量组分别给予不同浓度DFP溶液(13.72、27.44和54.88 mg/kg),每天1次。测定脑组织中Gly、GABA及铝、铜、镁、钙和铁的含量。结果与阴性对照组比较,铝单独染毒组和铝+各剂量DFP组大鼠脑组织中Gly和GABA的含量均较高,差异有统计学意义(P<0.01)。与铝单独染毒组比较,铝+各剂量DFP组大鼠脑组织中Gly和GABA的含量均较低,差异有统计学意义(P<0.01)。与阴性对照组比较,铝单独染毒组和铝+低、中剂量DFP组大鼠脑组织中铝含量均较高,差异有统计学意义(P<0.01)。与铝单独染毒组比较,铝+各剂量DFP组大鼠脑组织中铝含量均较低,差异有统计学意义(P<0.01);且随着DFP剂量的升高,大鼠脑组织中的铝含量呈下降趋势。各组大鼠脑组织中铜、镁、钙和铁含量间比较,差异均无统计学意义。结论 DFP可通过促进铝排出,恢复铝染毒大鼠脑组织中Gly和GABA的正常代谢,且对铜、镁、钙、铁等必需元素在脑中平衡不产生影响,对中枢神经系统具有明显保护作用。
Objective To investigate the effects of defriprone (DFP) on the inhibitory amino acid neurotransmitter glycine (Gly) and γ-aminobutyric acid (GABA) in the brain of aluminum-exposed rats. Methods 35 healthy Wistar male rats were randomly divided into 5 groups according to body weight: negative control group, aluminum alone exposure group and aluminum + low, middle and high dose DFP group, with 7 rats in each group. Mode of exposure, once daily, weekly continuous exposure to 5 d, an interval of 2 d. The first 8 weeks, the negative control group was given normal saline (1 ml / d), the other 4 groups were given AlCl3 solution (354.7 mg / kg). After 2 weeks, the rats in the negative control group and the aluminum-exposed group were given normal saline (1 ml / d), and the aluminum, DFP low, middle and high dose groups were given different concentrations of DFP solution (13.72, 27.44 and 54.88 mg / kg) 1 time. The content of Gly, GABA, aluminum, copper, magnesium, calcium and iron in brain tissue were measured. Results Compared with the negative control group, the contents of Gly and GABA in the brain of rats exposed to aluminum and DFP alone were significantly higher (P <0.01). Compared with aluminum alone group, the content of Gly and GABA in brain tissue of aluminum + DFP group at each dose was lower, the difference was statistically significant (P <0.01). Compared with the negative control group, the content of aluminum in the brain of rats exposed to aluminum alone and medium + low dose of DFP were higher than that of the negative control group, the difference was statistically significant (P <0.01). Compared with the aluminum-alone exposure group, the content of aluminum in the brain of the DFP group was lower than that of the aluminum alone group (P <0.01), and with the increase of DFP dose, The aluminum content is on the decline. The contents of copper, magnesium, calcium and iron in brain tissue of each group were not significantly different. Conclusion DFP can restore the normal metabolism of Gly and GABA in the brain of aluminum-exposed rats by promoting the excretion of aluminum, and has no effect on the balance of essential elements such as copper, magnesium, calcium and iron in the brain, and has obvious effects on central nervous system Protective effects.