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目的:探讨11β-羟基类固醇脱氢酶(11β-HSD)与促炎性细胞因子在川崎病(KD)发生、发展中的变化及意义。方法:应用Real-time PCR检测KD患儿急性期及治疗后外周血单核细胞内11β-HSD mRNA的表达,应用免疫印迹方法检测11β-HSD蛋白的表达;应用酶联免疫吸附试验检测血浆IL-17A和IL-6水平。结果:病例组患儿治疗前外周血单核细胞内11β-HSD1 mRNA表达水平及IL-17A和IL-6水平分别为7.13±0.79、(43.40±5.20)pg/m L、(68.30±6.26)pg/m L,较正常对照组1.00±0.07、(24.30±2.26)pg/m L、(30.04±2.86)pg/m L明显升高(P<0.01),而治疗后结果分别为3.43±0.52、(27.30±2.50)pg/m L、(38.30±3.50)pg/m L,均较治疗前明显下降(P<0.01);治疗前11β-HSD2 mRNA表达水平为0.32±0.05,明显低于正常对照组1.00±0.06(P<0.01),治疗后水平为0.82±0.04,较治疗前明显升高(P<0.01)。免疫印迹检测11β-HSD蛋白表达水平的结果分析与11β-HSD mRNA表达水平结果分析一致。结论:KD急性期11β-HSD、促炎性细胞因子在调节KD炎症反应中发挥着重要作用。
Objective: To investigate the changes and significance of 11β-hydroxysteroid dehydrogenase (11β-HSD) and pro-inflammatory cytokines in the pathogenesis and development of Kawasaki disease (KD). Methods: Real-time PCR was used to detect the expression of 11β-HSD mRNA in peripheral blood mononuclear cells of KD children at acute and post-treatment. The expression of 11β-HSD protein was detected by Western blotting. -17A and IL-6 levels. Results: The expression of 11β-HSD1 mRNA and the levels of IL-17A and IL-6 in peripheral blood mononuclear cells of patients before treatment were 7.13 ± 0.79, (43.40 ± 5.20) pg / m L, (68.30 ± 6.26) pg / m L were significantly higher than those in the normal control group (1.00 ± 0.07 vs. 24.30 ± 2.26 pg / m L, (30.04 ± 2.86) pg / m L respectively), while the results were 3.43 ± 0.52 , (27.30 ± 2.50) pg / m L, (38.30 ± 3.50) pg / m L, respectively, compared with those before treatment (P <0.01). The expression of 11β-HSD2 mRNA before treatment was 0.32 ± 0.05, The control group was 1.00 ± 0.06 (P <0.01), and the level after treatment was 0.82 ± 0.04, which was significantly higher than that before treatment (P <0.01). Western blot analysis of 11β-HSD protein expression level of the results of analysis and 11β-HSD mRNA expression level results of the analysis. Conclusion: KD acute 11β-HSD and proinflammatory cytokines play an important role in the regulation of KD inflammatory response.