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目的:了解EB病毒(EBV)感染与患儿免疫功能的关系。方法:选取2010年1月至2015年12月我院儿科收治的EBV感染儿童80例(EBV感染组)以及同期在我院体检的正常儿童38例(对照组),采用散射比浊法检测血清免疫球蛋白和补体水平,采用流式细胞仪检测外周血淋巴细胞分化抗原T淋巴细胞(CD3~+)、辅助性T细胞(CD4~+)、细胞毒性T细胞(CD8~+)、B淋巴细胞(CD19~+)及NK细胞(CD56~+)阳性率以及CD4~+/CD8~+值。结果:两组IgA水平比较差异无统计学意义(P>0.05)。EBV感染组IgM、IgG、IgE、C3和C4表达水平均升高,与对照组比较差异均有统计学意义(P<0.05)。EBV感染组CD3~+、CD8~+、CD19~+、CD56~+计数高于对照组,CD4~+计数、CD4~+/CD8~+值低于对照组,差异有统计学意义(P<0.05)。结论:EBV感染与患儿的免疫功能相关,与CD4~+T细胞和B细胞计数减少有明显的关系,可以采用外周血淋巴细胞分化抗原测定方法检测EBV感染后的淋巴细胞亚群变化。
Objective: To understand the relationship between Epstein-Barr virus (EBV) infection and children’s immune function. Methods: From January 2010 to December 2015, 80 children with EBV infection (EBV infection group) and 38 normal children (control group) undergoing pediatric physical examination in our hospital were enrolled in this study. Serum was detected by nephelometry Immunoglobulin and complement levels were detected by flow cytometry. The levels of peripheral blood lymphocyte differentiation antigen T lymphocytes (CD3 ~ +), helper T cells (CD4 ~ +), cytotoxic T lymphocytes (CD8 ~ +) (CD19 ~ +) and NK cells (CD56 ~ +) positive rate and CD4 ~ + / CD8 ~ + value. Results: There was no significant difference in IgA levels between the two groups (P> 0.05). The expression levels of IgM, IgG, IgE, C3 and C4 in EBV infection group were significantly higher than those in control group (P <0.05). The counts of CD3 ~ +, CD8 ~ +, CD19 ~ + and CD56 ~ + in EBV infection group were higher than those in control group. The CD4 ~ + counts and CD4 ~ + / CD8 ~ 0.05). Conclusions: EBV infection is related to the immune function of children and has a clear relationship with the decrease of CD4 ~ + T cells and B cell count. Peripheral blood lymphocyte differentiation antigen assay can be used to detect the changes of lymphocyte subsets after EBV infection.