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目的建立细梗香草药材中两种皂苷的含量测定法。方法采用HPLC-ELSD,以乙腈和0.3%醋酸溶液梯度洗脱,流速1.0 mL.min-1;漂移管温度100℃,气体流速2.6 L.min-1。结果细梗香草药材中细梗香草皂苷B和细梗香草皂苷C分别在0.824 0～8.240 0μg(r=0.999 8)和0.404 8～4.048 0μg(r=0.999 8)内线性关系良好;平均加样回收率分别为101.29%(n=6,RSD=1.60%)和99.62%(n=6,RSD=2.16%)。不同时期不同产地药材的皂苷含量存在差异。结论本法操作简便、灵敏度高、重现性好,可用于细梗香草药材的质量控制。
Objective To establish a method for the determination of two saponins in the stems and herbs of Sophora japonica. Methods The HPLC-ELSD was used to elute gradient acetonitrile and 0.3% acetic acid with a flow rate of 1.0 mL · min-1. The drift tube temperature was 100 ℃ and the gas flow rate was 2.6 L.min-1. Results The results showed that there was a good linear relationship between H. tuberosum B and S. japonicum glycosides C in the range of 0.824 0-8.240 0μg (r = 0.999 8) and 0.404 8-4.048 0μg (r = 0.999 8) The recoveries were 101.29% (n = 6, RSD = 1.60%) and 99.62% (n = 6, RSD = 2.16%), respectively. The saponin contents of medicinal materials from different areas in different periods differed. Conclusion This method is simple, sensitive and reproducible. It can be used for the quality control of the finely chopped herbs.