目的:建立黄精中小分子糖的含量测定方法,研究其最佳提取工艺。方法:采用苯酚-硫酸法测定小分子糖含量,以小分子糖含量为考察指标,通过单因素试验和正交试验法确定黄精中小分子糖的最佳提取工艺。结果:最佳提取工艺条件为提取温度90℃,料液比1∶30,提取时间2 h。结论:该工艺简便、合理、小分子糖提取率较高。 OBJECTIVE: To establish a method for the determination of small and middle molecular weight of Polygonatum sibiricum and to study its optimum extraction process. Methods: The content of small molecule sugar was determined by the method of phenol - sulfuric acid, and the content of small molecule sugar was taken as the index. The optimum extraction technology of small molecule sugar was determined by single factor test and orthogonal test. Results: The optimal extraction conditions were extraction temperature 90 ℃, material-liquid ratio 1:30, extraction time 2 h. Conclusion: The process is simple and reasonable, and the extraction rate of small molecule sugar is high.