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目的研究自行设计的新型青光眼房水引流物在兔眼上的生物相容性。方法新西兰大白兔6只,每只兔随机取一眼作为实验眼,另一眼作为对照眼。实验眼行巩膜瓣下植入聚砜引流管,一端植入前房0.5 cm;对照组眼单纯做巩膜瓣。术前和术后的1、2、4周,测量眼压并观察眼前节形态,术后1周进行超声生物显微镜检查,术后第1、2、4周分别取2只兔,摘除双侧实验眼与对照眼各2只,行病理HE染色检查,观察兔眼局部组织对植入物的反应。结果术后第1天,实验眼和对照眼角膜透明,50%试验眼前房出现Tydall征(+),12.5%对照眼前房出现Tydall征(+),所有眼均未见纤维素性渗出。术后1周,实验眼与对照眼Tydall征均为(-)。实验眼与对照眼术后早期的病理组织学改变相似,都表现为炎症细胞浸润,成纤维细胞逐渐增多,未见异物巨细胞。结论聚砜引流管具有良好的生物相容性,植入兔眼后未见局部组织排斥反应。
Objective To study the biocompatibility of a new type of glaucoma aqueous drainage designed by ourselves. Methods Six New Zealand white rabbits were randomly selected. Each rabbit was randomly selected as the experimental eye and the other as the control eye. Experimental eyes were scleral flap implanted polysulfone drainage tube, 0.5 cm anterior chamber was implanted; the control group of eyes simply scleral flap. Before and 1, 2, and 4 weeks after operation, the intraocular pressure was measured and the anterior segment morphology was observed. Ultrasound biomicroscopy was performed one week after operation. Two rabbits were removed at 1, 2 and 4 weeks after operation, Two experimental eyes and two control eyes were examined by pathological HE staining to observe the response of local tissues of rabbits to the implants. Results On the first postoperative day, the experimental and control corneas were clear, Tydall’s sign (+) appeared in 50% of test eyes and Tydall’s sign (+) in 12.5% of control eyes. No cellulolytic exudate was observed in all eyes. One week after operation, Tydall sign was both (-) in experimental and control eyes. The histopathological changes of the experimental eye and the control eye were similar in the early postoperative period, which showed infiltration of inflammatory cells and gradual increase of fibroblasts with no giant cells of foreign bodies. Conclusion Polysulfone drainage tube has a good biocompatibility, no local tissue rejection after implantation in rabbit eyes.