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目的:从神经内分泌免疫网络(NEI)角度研究脾虚痰浊证巴马小型猪脑肠肽和神经递质的影响。方法:将巴马小型猪随机分为正常组和模型组,施以每日冲刺跑步训练联合高脂单笼饲养干预,建立小猪脾虚痰浊证模型。应用酶联免疫法(Elisa)测定血清中皮质醇(COR)、血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)、5-羟色胺(5-HT)、胰高血糖素样肽-1(GLP-1)、乙酰胆碱(Ach)和去甲肾上腺素(NE)水平。结果:与正常组比较,模型组小型猪脑肠肽、神经递质水平发生差异性变化。结论:脾虚痰浊证模型神经内分泌免疫网络中脑肠肽、神经递质失常,提示NEI是客观存在的。某一环节的失衡可能是出现脾虚痰浊证的病理机制之一。
Objective: To study the effect of neurotransmitter and ghrelin on the Bama miniature pigs with phlegm and phlegm syndrome of phlegm in spleen deficiency from the perspective of neuroendocrine and immune network (NEI). Methods: The Bama miniature pigs were randomly divided into normal group and model group. Sprint jogging training combined with high fat single cage feeding intervention was performed to establish the model of phlegm and phlegm syndrome of spleen deficiency in piglets. Serum levels of cortisol (COR), angiotensin II (AngⅡ), aldosterone (ALD), serotonin 5-HT, GLP- 1), acetylcholine (Ach) and norepinephrine (NE) levels. Results: Compared with the normal group, the levels of brain gut peptide and neurotransmitter in the miniature pigs in the model group changed significantly. Conclusion: The neuropeptide and neurotransmitter in neuroendocrine-immune network of phlegm and phlegm syndrome model of spleen deficiency syndrome suggest NEI is an objective reality. One part of the imbalance may be one of the pathological mechanisms of phlegm phlegm syndrome.