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为提高郁金香鳞茎愈伤组织的诱导频率,以郁金香栽培品种“Gudoshnik”的鳞茎为材料,对影响郁金香鳞茎愈伤组织形成的几个主要因素进行了研究.结果表明:采用内层鳞片作外植体,在MS+NAA2.0mg/L+BA2.0mg/L+0.2%活性炭的培养基上,黑暗处理5~10d,可大大提高鳞茎愈伤组织的诱导率,其诱导率可达53%.
In order to improve the induction frequency of bulb callus of tulip, the main factors affecting the bulb callus formation of tulip were studied using bulbs of tulip cultivars “Gudoshnik” as materials. The results showed that the callus induction of bulb callus could be greatly enhanced by using the inner scale as explant on medium of MS + NAA2.0mg / L + BA2.0mg / L + 0.2% activated carbon for 5 ~ 10d. Rate up to 53%.