新鲜组织恒冷切片固定后再进行孵育,是防止酶扩散的重要方法之一。但固定剂对酶活性有抑制作用,而有关固定切片对酶活性的影响报道甚少。为孵育前切片固定时选择固定剂提供科学依据,我们进行了本实验。 实验用大鼠肝、肾组织恒冷切片(8μm),在4℃下分别用4％戊二醛、4％缓冲甲醛和4％多聚甲醛(均用pH7.4,0.2M磷酸缓冲液配制)固定液及纯丙酮,固定不同时间(3、10、20、30分钟),然后作酶反应。选用一般认 Fresh tissue frozen section fixed and then incubated, is an important method to prevent the proliferation of enzymes. However, the immobilizing agent has an inhibitory effect on the enzyme activity, and the effect of the fixed slice on the enzyme activity is poorly reported. To provide a scientific basis for the selection of fixatives for fixation before incubation, we performed this experiment. Experimental rat liver and kidney tissue sections were cryostatically frozen (8μm), respectively, at 4 ° C with 4% glutaraldehyde, 4% buffered formaldehyde and 4% paraformaldehyde (both pH7.4, 0.2M phosphate buffer ) Fixed solution and acetone, fixed at different times (3,10,20,30 minutes), and then for enzyme reaction. Use the general admission