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利用抗体捕获法 ,从表面展示随机肽序列的噬菌体文库中筛选到与志贺毒素B亚基 (StxB)结合 ,并能抑制志贺毒素细胞毒效应的噬菌体克隆 ;依据其中 1个克隆序列 (A12 )合成的肽可以与志贺毒素的受体Gb3竞争结合StxB ,并抑制志贺毒素(Stx)的细胞毒和肠毒活性 ;抑制 5×CD50 剂量的Stx细胞毒效应需 2 2 .7μmol的A12合成肽 .筛选得到的 2个噬菌体克隆 (A3 ,A12 )编码的氨基酸序列不同 ,但能竞争结合StxB ,推测它们形成相同或相似的空间结构 .为志贺毒素抑制剂进一步研究打下基础 ,对其他相关药物的研制亦有参考价值 .
Phage clones that bind to Shiga toxin B subunit (StxB) and inhibit the cytotoxic effect of Shiga toxin were screened from the phage library showing random peptide sequences by antibody capture. Based on the results of one cloned sequence (A12 ) Synthesized peptides competed with Shigatoxin receptor Gb3 for binding to StxB and inhibited the cytotoxic and enterotoxic activity of Shiga toxin (Stx); Stx cytotoxicity to inhibit 5xCD50 dose required 22.7 μmol of A12 The two phage clones (A3 and A12) screened for different amino acid sequences but competed for binding to StxB, suggesting that they form the same or similar spatial structure, which laid the foundation for the further study of Shiga toxin inhibitors, The development of related drugs also have reference value.