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目的:通过研究安律胶囊对豚鼠心室肌细胞钾离子通道的影响,以探讨其抗心律失常的作用机制。方法:采用膜片钳全细胞记录技术记录了安律胶囊对单个豚鼠心室肌细胞钾电流的影响。结果:①安律胶囊清膏2、20、200μg/L可使IK1幅值从给药前的(-2.06±0.29,nA)降低到(-1.95±0.34、-1.67±0.43和-1.62±0.25,nA),大剂量具有显著的统计学意义(P<0.05);在对IK1时效关系的实验中,在200μg/L的安律胶囊清膏作用下,未计时时最大内向峰值钾电流为(-1.60±0.27,nA),1min、4min、8min后,最大内向峰值钾电流分别为(-1.56±0.35,-1.34±0.26和-1.26±0.29,nA),无统计学意义(P>0.05)。②安律胶囊清膏2、20、200μg/L可使Ito1幅值从未给药时的(1.14±0.17,nA),降低到(1.13±0.17、1.10±0.16和1.06±0.13,nA),但无统计学意义(P>0.05)。③安律胶囊清膏2、20、200μg/L可剂量依赖性的降低Ik,分别使Ik幅值从给药前的(1.45±0.15,nA),降低到(1.42±0.15、1.29±0.10和1.20±0.11,nA),中剂量组具有显著的统计学意义(P<0.05),大剂量具有极为显著的统计学意义(P<0.01);在对Ik时效关系的实验中,在200μg/L的安律胶囊清膏作用下,未计时时最大内向峰值电流为(1.45±0.15,nA),1min、4min、8min后,最大内向峰值钾电流分别为(1.21±0.10,1.20±0.12和1.11±0.13,nA),无统计学意义(P>0.05)。结论:安律胶囊对IK1有一定的阻滞作用且可剂量依赖性的阻滞Ik,但不具有时间依赖性,对Ito1无阻滞作用,这是其抗心律失常作用的机理之一。
OBJECTIVE: To study the effect of Arrheni capsule on potassium channel in guinea pig ventricular myocytes and to explore its anti-arrhythmic mechanism. Methods: Whole-cell patch-clamp recording technique was used to record the effect of AnLi capsule on potassium current in isolated guinea pig ventricular myocytes. Results: (1) Allic capsule clear extract 2, 20, 200μg / L reduced the amplitude of IK1 from (-2.06 ± 0.29, nA) before administration to (-1.95 ± 0.34, -1.67 ± 0.43 and -1.62 ± 0.25 , nA) and high dose (P <0.05). In the experiment of the relationship of IK1 aging time, under the action of 200μg / L Anlu capsule cream, the maximum inward peak potassium current was (-1.56 ± 0.35, -1.34 ± 0.26 and -1.26 ± 0.29, nA), -1.60 ± 0.27, nA, 1min, 4min and 8min, respectively. There was no statistical significance (P> 0.05) . (2) Anlu capsule clear paste (2,20,200μg / L) decreased the amplitude of Ito1 from (1.14 ± 0.17, nA) to (1.13 ± 0.17,1.10 ± 0.16 and 1.06 ± 0.13, nA) But not statistically significant (P> 0.05). (3) Allic capsule clear paste 2,20,200μg / L dose-dependently reduced Ik, Ik amplitude decreased from 1.45 ± 0.15, nA before administration to 1.42 ± 0.15,1.29 ± 0.10 and 1.20 ± 0.11, nA), the middle dose group had significant statistical significance (P <0.05), and the high dose had the extremely significant statistical significance (P <0.01). In the experiment of Ik aging, (1.45 ± 0.15, nA), 1min, 4min and 8min after unloading, the maximum inward peak potassium currents were (1.21 ± 0.10,1.20 ± 0.12 and 1.11 ± 0.13, nA), no statistical significance (P> 0.05). CONCLUSION: Allic capsule has a certain block effect on IK1 and can block Ik in a dose-dependent manner, but it has no time-dependent effect and has no block effect on Ito1, which is one of its antiarrhythmic mechanisms.