论文部分内容阅读
目的探讨快速眼动(REM)睡眠剥夺(SD)对大鼠脑干和海马大麻素CB-1受体的影响。方法采用改良多平台睡眠剥夺法建立REM睡眠剥夺模型。将40只Sprague-Dawley大鼠随机分为空白对照组(CC)、环境对照组(TC),以及睡眠剥夺1d(SD1d)、3d(SD3d)和5d(SD5d)组,每组8只。应用逆转录-聚合酶链反应方法检测大鼠脑干及海马CB-1受体mRNA的表达,电镜观察其超微结构的改变。结果SD各组大鼠脑干与海马超微结构均有神经元凋亡,SD3d和SD5d组尤著。(1)脑干CB-1受体mRNA表达值SD1d组(0.789±0.139)和SD3d组(1.264±0.182)均高于CC组(0.420±0.054),且SD3d组高于SD1d组(P<0.05),SD5d组(0.678±0.145)与CC组的差异无统计学意义(P>0.05)。(2)海马CB-1受体mRNA表达值SD1d组(0.598±0.098)高于CC组(0.374±0.064),SD3d组(0.258±0.072)低于CC组,且SD3d组低于SD1d组(P<0.05);SD5d组(0.448±0.177)与CC组的差异无统计学意义(P>0.05)。结论REM睡眠剥夺能造成脑干及海马神经元的损伤,在不同睡眠剥夺时期CB-1受体mRNA表达不同,其中CB-1受体表达增强可能是一种自身稳定调节的保护机制。
Objective To investigate the effects of REM sleep deprivation (SD) on CB-1 receptor in brain stem and hippocampus of rats. Methods REM sleep deprivation model was established by modified multi-platform sleep deprivation method. Forty Sprague-Dawley rats were randomly divided into blank control group (CC), environmental control group (TC) and sleep deprivation 1d (SD1d), 3d (SD3d) and 5d (SD5d) groups. The expression of CB-1 receptor mRNA in rat brainstem and hippocampus was detected by reverse transcription-polymerase chain reaction (RT-PCR). The ultrastructural changes were observed by electron microscopy. Results SD rat brain and hippocampal ultrastructure were neuronal apoptosis, especially SD3d and SD5d group. (1) The expression of CB-1 receptor mRNA in brain stem of SD rats was significantly higher in SD1d group (0.789 ± 0.139) and SD3d group (1.264 ± 0.182) than that in CC group (0.420 ± 0.054) ), But there was no significant difference between SD5d group (0.678 ± 0.145) and CC group (P> 0.05). (2) Hippocampal CB-1 receptor mRNA expression in SD1d group (0.598 ± 0.098) was significantly higher than that in CC group (0.374 ± 0.064), SD3d group (0.258 ± 0.072) was lower than CC group, and SD3d group was lower than SD1d group <0.05). There was no significant difference between SD5d group (0.448 ± 0.177) and CC group (P> 0.05). Conclusion REM sleep deprivation can cause damage to the brainstem and hippocampal neurons. CB-1 receptor mRNA expression is different during different sleep deprivation periods. Enhanced expression of CB-1 receptor may be a self-regulated regulatory mechanism.