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目的:探讨银杏叶提取物对血管内皮细胞的保护作用及可能的保护机制。方法:进行血管内皮细胞培养。用过氧化氢(H2O2)处理血管内皮细胞建立细胞凋亡模型。将细胞分为四组:空白对照组、H2O2处理组、单独银杏叶提取物处理组、银杏叶提取物预处理组(提前2h给药后H2O2处理)。进行MTT检测细胞的相对活力、RT-PCR检测目的基因CHOP的表达、Western Blot分析目的蛋白CHOP的表达等。结果:与对照处理组比较,H2O2处理组细胞凋亡率、CHOPmRNA相对表达及CHOP蛋白表达量明显升高。与H2O2处理组比较,银杏叶提取物预处理组细胞凋亡指数、CHOPmRNA相对表达及CHOP蛋白表达量明显降低(P<0.05)。结论:作为一种清除自由基、抗氧化、抗衰老药物,银杏叶提取物可能通过调节CHOP蛋白选择性地抑制过度的内质网应激来保护血管内皮细胞。
Objective: To investigate the protective effect of Ginkgo biloba extract on vascular endothelial cells and its protective mechanism. Methods: Vascular endothelial cell culture was performed. Apoptosis model was established by treating vascular endothelial cells with hydrogen peroxide (H2O2). The cells were divided into four groups: blank control group, H2O2 treatment group, Ginkgo biloba extract alone treatment group, Ginkgo biloba extract pretreatment group (H2O2 treatment after 2h administration). The relative viability of cells was detected by MTT. The expression of CHOP was detected by RT-PCR and the expression of CHOP by Western Blot. Results: Compared with the control group, the apoptosis rate, the relative expression of CHOP mRNA and the expression of CHOP protein in H2O2-treated group were significantly increased. Compared with H2O2 treatment group, the apoptosis index, the relative expression of CHOP mRNA and the expression of CHOP protein in Ginkgo biloba extract pretreatment group were significantly decreased (P <0.05). CONCLUSION: Ginkgo biloba extract, as a scavenging free radical, anti-oxidant and anti-aging drug, may protect vascular endothelial cells by regulating CHOP protein selectively to inhibit excessive endoplasmic reticulum stress.