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目的 探讨中药柴胡对急性肝脏缺血性损伤的保护作用。 方法 大鼠56只随机分为正常组(n=8)、普食组(n=24)和柴胡组(n=24),采用常温下肝门血流完全阻断60min制备大鼠肝脏缺血性损伤模型,动态检测术后血清丙氨酸转氨酶(ALT)、谷草氨酸转氨酶(AST)及肝组织5′-核苷酸酶(5′-NT)活性的变化。 结果 术后1d,各组大鼠血清ALT,AST(kU/L,(?)±s)均明显高于正常(ALT:普食组116±18或柴胡组105±18 vs 正常22±8;AST:192±25或175±23 vs 26±8,P<0.05);而5′-NT(U/g)活性明显低于正常(0.65±0.03或0.68±0.04 vs 1.65±0.09,P<0.05);术后4d,实验组上述指标虽部分恢复,但仍未达正常水平;其中普食组较柴胡组恢复慢,且有明显差异(普食组vs柴胡组,ALT 107±15 vs 62±12,AST 167±20 vs 71±14,5′-NT 0.82±0.04 vs 1.12±0.07,P<0.05);术后7d,柴胡组ALT,AST均恢复正常,而普食组尚未正常,柴胡组肝组织5′-NT(1.01±0.05 vs 1.50±0.08,P<0.05)明显高于普食组。 结论 柴胡对肝脏缺血性损伤有保护作用,能降低血清转氨酶、保护肝功能、抑制肝损害,为临床上应用柴胡治疗肝脏手术后肝功能损害,促进肝功能恢复奠定了实验基础。
Objective To explore the protective effect of Bupleurum on acute hepatic ischemia injury. METHODS: 56 rats were randomly divided into normal group (n=8), normal food group (n=24), and bupleurum group (n=24). Rat hepatic plexus was prepared by blocking hepatic portal vein flow at room temperature for 60 min. Blood-alveolar injury model was used to dynamically detect the changes of serum alanine aminotransferase (ALT), glutamate transaminase (AST) and liver tissue 5′-nucleotidase (5′-NT) activity. Results On the 1st postoperative day, serum ALT, AST (kU/L, (?)±s) were significantly higher than normal in all groups (ALT: normal food group 116±18 or 105±18 vs. normal 22±8 in Chaihu group). AST: 192±25 or 175±23 vs 26±8, P<0.05); while 5′-NT(U/g) activity was significantly lower than normal (0.65±0.03 or 0.68±0.04 vs 1.65±0.09, P< 0.05); 4 days after surgery, although the above indicators in the experimental group partially recovered, but still not reach the normal level; which general food group than the Chaihu group, the recovery is slow, and there are significant differences (Pu food group vs. Bupleurum group, ALT 107 ± 15 Vs 62±12, AST 167±20 vs 71±14,5′-NT 0.82±0.04 vs 1.12±0.07, P<0.05); 7d postoperatively, ALT and AST in the Bupleurum group returned to normal, but the general food group has not yet Normal, 5′-NT (1.01±0.05 vs 1.50±0.08, P<0.05) in the liver tissue of the Bupleurum was significantly higher than that of the normal food group. Conclusion Bupleurum has a protective effect on hepatic ischemic injury, can reduce serum transaminases, protect liver function, inhibit liver damage, and lay a foundation for the clinical application of Radix Bupleuri in the treatment of liver damage after hepatic surgery and promote liver function recovery.