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应用聚合酶链式反应—单链构像多态性分析（ＰＣＲ－ＳＳＣＰ）银染色法检测了３９例膀胱移行上皮癌组织中ｐ５３基因的突变率为３０．８％，其中５、６、７、８外显子的突变率分别为７．７％、５．１％、５．１％、１２．８％；病理分级的Ⅰ、Ⅱ、Ⅲ级肿瘤组织中ｐ５３基因突变率分别为７．１％、３０．０％、５３．３％（Ｐ＜０．０５）。提示ｐ５３基因突变在膀胱肿瘤的衍变过程中可能起重要作用，可能是致其恶性程度提高的继发事件；ＰＣＲ－ＳＳＣＰ银染法是一种快速、简单且安全的检测基因突变的方法，具有推广应用价值。
Polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP) silver staining was used to detect the mutation rate of p53 gene in 39 cases of transitional cell carcinoma of the bladder with a mutation rate of 30.8%, among which, 5,6,7 The mutation rates of exon 8 and exon 8 were 7.7%, 5.1%, 5.1% and 12.8% respectively. The mutation rates of p53 gene in pathological grade Ⅰ, Ⅱ and Ⅲ tumors were 7 and 7 respectively. 1%, 30.0%, 53.3% (P <0.05). It is suggested that the mutation of p53 gene may play an important role in the carcinogenesis of bladder neoplasm and may be a secondary event leading to the increase of malignant degree. PCR-SSCP silver staining is a rapid, simple and safe method to detect gene mutation, Promote the value of application.