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目的探讨不同浓度氟对大鼠肝功和谷胱苷肽过氧化物酶(GSH-Px)基因表达及肝细胞超微结构影响。方法在饮水中加入不同剂量氟化钠喂饲大鼠30 d后,测定血清氟含量、门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活力、肝组织中GSH-Px活性及mRNA表达,观察大鼠肝细胞超微结构。结果随染氟剂量增加,各剂量组大鼠血氟浓度逐渐增加,血清中AST、ALT含量逐渐增加,呈剂量-效应关系(R=0.889,R=0.729,P<0.01);GSH-Px酶活力单位从(19.672±1.025)下降至(11.777±1.529),与对照组比较,差异有统计学意义(P<0.01);GSH-Px的mRNA表达下降(P<0.05)。中、高剂量组大鼠肝细胞超微结构可见线粒体肿胀,有较多脂肪变性。结论过量氟可能诱导GSH-Px的mRNA表达下降,使大鼠肝脏抗氧化系统受到抑制。
Objective To investigate the effects of different concentrations of fluoride on hepatic and glutathione peroxidase (GSH-Px) gene expression and hepatocyte ultrastructure in rats. Methods After the rats were fed with different doses of sodium fluoride for 30 days in the drinking water, the levels of serum fluoride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), hepatic GSH- Px activity and mRNA expression were observed in rat liver cell ultrastructure. Results With the increase of fluoride dose, the blood fluoride levels gradually increased and the levels of AST and ALT gradually increased in dose-dependent manner (R = 0.889, R = 0.729, P <0.01). The levels of GSH-Px (19.672 ± 1.025) decreased to (11.777 ± 1.529), the difference was statistically significant (P <0.01), and the mRNA expression of GSH-Px was decreased (P <0.05). Medium and high dose groups of rat hepatocytes ultrastructure showed mitochondrial swelling, more fatty degeneration. Conclusion Excessive fluoride may decrease the mRNA expression of GSH-Px, and inhibit the hepatic antioxidant system.