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应用PCR- SSCP、Northern blot和免疫细胞化学方法,对30例临床各期慢性粒细胞性白血病(CML)和K562、HL-60细胞系进行p53基因突变与表达的研究。结果发现,17例慢性期CML中仪1例有外显子6的突变和细胞内P53蛋白的聚积,所有病例均可检测到p53的mRNA表达。而4例加速期和9例急变期CML中各有2例分别在外显子5、6、7上发现突变,有p53表达的仅7例,1例加速期患者的P53蛋白呈强阳性。K562和HL-60细胞系中P53蛋白和mRNA均呈阴性。CML恶化进程中p53基因突变和表达频率的改变表明P53基因的改变至少在部分CML急变中起着重要作用。
Using PCR-SSCP, Northern blot and immunocytochemistry, p53 gene mutation and expression were studied in 30 clinical stages of chronic myelogenous leukemia (CML) and K562 and HL-60 cell lines. The results showed that 17 cases of CML in the chronic phase had exon 6 mutations and accumulation of intracellular P53 protein, and p53 mRNA expression was detected in all cases. The mutations were found in exons 5, 6, and 7, respectively, in 4 cases of accelerated phase and 9 cases of blast crisis CML. Only 7 cases had p53 expression, and 1 case of accelerated phase showed strong positive P53 protein. Both P562 protein and mRNA were negative in K562 and HL-60 cell lines. Changes in the frequency of p53 gene mutation and expression in the process of deteriorating CML indicate that changes in the P53 gene play an important role in at least some of the CML blasts.