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产肠毒素性大肠杆菌(ETEC)是发展中国家儿童急性腹泻的主要病因,但对其传播和流行病学了解甚少,主要是难以从大肠杆菌菌株中区别产毒性或不产毒性。作者介绍一种新的膜滤纸法检测产LT大肠杆菌应用于流行病学研究,并与神经节苷-酶联免疫吸附试验(GMI-ELISA)和DNA-DNA杂交法进行比较。用取自Biken法收集的50株大肠杆菌菌株作研究。用源于临床患者的大肠杆菌487475菌株作LT-阳性对照和源于环境的大肠杆菌WF5菌株作LT-阴性对照。将细菌点种于一49mm醋酸纤维膜滤纸(孔径0.45μm)上,置于浸泡在选择性肉汤培养基中的一个吸收纸垫上。培养基的组成(以
Enterotoxigenic Escherichia coli (ETEC) is the leading cause of acute diarrhea in children in developing countries, but little is known about its spread and epidemiology, mainly because it is difficult to discriminate between virulent or non-toxigenic strains of E. coli. The authors describe a new membrane filter assay for the detection of E. coli producing LT for epidemiological studies and comparison with ganglioside-enzyme-linked immunosorbent assay (GMI-ELISA) and DNA-DNA hybridization. Fifty Escherichia coli strains collected from the Biken method were used for the study. The LT-positive control and the E. coli WF5 strain from the environment were used as LT-negative controls with E. coli 487475 strains from clinical patients. Bacteria were spotted on a 49 mm cellulose acetate membrane filter (pore size 0.45 μm) and placed on an absorbent pad soaked in selective broth. Medium composition (with