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目的:建立测定蝎龙接骨散活性成分血竭素含量的高效液相色谱法。方法:采用VP-ODS(250mm×4.6mm,5μm)色谱柱,以乙腈-0.05mol·L~(-1)磷酸二氢钠溶液(45:55)(用磷酸调pH为3.5)为流动相,流速为1.0ml·min~(-1),检测波长:440nm。结果:血竭素在9.59～47.94μg·ml~(-1)浓度范围内,峰面积与浓度线性关系良好,r=0.999 6,平均加样回收率为99.75%(RSD =0.99%)。结论:本法简便,准确,可用于蝎龙接骨散中血竭素的含量测定。
Objective: To establish a high performance liquid chromatography method for the determination of the dracorhodin content of Qilong Jiegu Powder. METHODS: A mobile phase composed of acetonitrile-0.05 mol·L -1 sodium dihydrogen phosphate (45:55) (pH adjusted to 3.5 with phosphoric acid) was used on a VP-ODS (250mm×4.6mm, 5μm) column. The flow rate was 1.0 ml·min -1 and the detection wavelength was 440 nm. Results: In the range of 9.59-47.94 μg·ml-1, the linear relationship between the peak area and concentration was good, r=0.999 6. The average recovery was 99.75% (RSD = 0.99%). Conclusion: This method is simple, accurate, and can be used for the determination of Dracorhodin in Qilong Jiegu Powder.