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垂体瘤转化基因(PTTG)具有促进体外细胞转化和体内致瘤的能力,在乳腺癌组织中高表达,并与乳腺癌的复发和淋巴结转移有关.但PTTG能否通过调节基质金属蛋白酶(MMP-2、MMP-9)调控乳腺癌的侵袭与转移尚不清楚.本研究证明,PTTG可能因促进乳腺癌细胞中MMP-2、MMP-9分泌而在乳腺癌细胞侵袭、转移中发挥重要作用.免疫组织化学PV 9000通用型两步法显示,60例乳腺浸润性导管癌组织中,PTTG、MMP-2和MMP-9表达定位于肿瘤细胞胞浆,阳性率与周围正常乳腺组织相比,差异均具有统计学意义(P<0.05).三者阳性表达均与淋巴结转移及TNM分期有关(P<0.05);与患者年龄、肿瘤大小等无关(P>0.05).乳腺浸润性导管癌中PTTG分别与MMP-2、MMP-9的表达呈正相关(P<0.05).小RNA干扰技术干扰乳腺癌细胞株MDA-MB-231中的PTTG,Western印迹结果显示,干扰组与对照组相比,PTTG、MMP-2和MMP-9蛋白的表达水平明显下降.Transwell侵袭实验显示,干扰组肿瘤细胞体外侵袭能力明显降低(P<0.01).本研究表明,PTTG可能通过促进乳腺癌细胞中MMP-2、MMP-9分泌,促进乳腺癌细胞的侵袭、转移.
Pituitary tumor transforming gene (PTTG) has the ability to promote cell transformation in vitro and tumorigenicity in vivo, which is highly expressed in breast cancer tissues and is related to the recurrence and lymph node metastasis of breast cancer.However, PTTG can regulate the expression of matrix metalloproteinase , MMP-9) to regulate the invasion and metastasis of breast cancer is unclear.This study demonstrates that PTTG may play an important role in the invasion and metastasis of breast cancer cells by promoting the secretion of MMP-2 and MMP-9 in breast cancer cells. The histochemistry PV 9000 universal two-step method showed that the expression of PTTG, MMP-2 and MMP-9 localized in the cytoplasm of tumor cells in 60 cases of invasive ductal breast cancer, the positive rate was significantly lower than that in the normal breast tissues (P <0.05) .The positive expression of the three was related to the lymph node metastasis and TNM staging (P <0.05), but not to the patient’s age, tumor size, etc. (P> 0.05). PTTG in invasive ductal carcinoma of breast (P <0.05) .The expression of MMP-2 and MMP-9 were positively correlated (P <0.05) .The results of Western blot showed that the expression of PTTG in the interference group was lower than that in the control group , MMP-2 and MMP-9 protein expression decreased significantly .Transwel Invasion experiments showed that the invasion ability of tumor cells in the interference group was significantly reduced (P <0.01) .This study shows that PTTG may promote the invasion and metastasis of breast cancer cells by promoting the secretion of MMP-2 and MMP-9 in breast cancer cells.