论文部分内容阅读
利用含p Ri A4质粒的农杆菌C58C1浸染白英叶片获得能在无植物生长物质培养基上自主生长的毛状根单克隆系,且毛状根中薯蓣皂苷元含量高于野生型白英植株。在液体培养基中添加不同浓度的6-苄氨基腺嘌呤(6-BA)、萘乙酸(NAA)或6-BA与NAA的结合处理白英毛状根,观察其对毛状根生长的影响,并利用高效液相色谱法(HPLC)测定毛状根中薯蓣皂苷元含量。结果表明,6-BA抑制白英毛状根的生长,且随6-BA浓度增加,抑制作用越明显,6-BA能增加白英毛状根每克干物质中薯蓣皂苷元的含量,但降低培养瓶中毛状根薯蓣皂苷元总量。NAA对毛状根的生长有促进作用,其中,经0.5 mg·L-1NAA处理的毛状根在培养30 d时干重达到同期对照的1.76倍。虽然NAA降低每克干物质中薯蓣皂苷元含量,但提高培养瓶中毛状根薯蓣皂苷元总量,薯蓣皂苷元总量最高可达0.97 mg(DW)·瓶-1,是对照整个培养过程中最大值的1.37倍。6-BA与0.5 mg·L-1 NAA的结合抑制白英毛状根的生长,同时降低每克干物质中薯蓣皂苷元的含量。
The hairy root monoclone which could grow autonomously on plant growth-free medium was obtained by using Agrobacterium C58C1 harboring p Ri A4 plasmid to infect white-leaf plants. The content of diosgenin in hairy roots was higher than that of wild-type plants . The effects of 6-BA, NAA or 6-BA and NAA on hairy root growth were observed in liquid medium The contents of diosgenin in hairy roots were determined by high performance liquid chromatography (HPLC). The results showed that 6-BA inhibited the growth of white hairy roots, and the more the 6-BA concentration was, the more obvious the inhibition was. 6-BA increased the content of diosgenin per gram of dry matter of white hairy roots Reduce the total amount of hairy root diosgenin in the flask. NAA promoted the growth of hairy roots, and the hairy roots treated with 0.5 mg · L -1 NAA reached 1.76 times the dry weight of the control at 30 days after culture. Although NAA reduced the content of diosgenin per gram of dry matter, increasing the total amount of hairy root diosgenin in the flask resulted in a total diosgenin content of up to 0.97 mg (DW) 1.37 times the maximum value. The combination of 6-BA and 0.5 mg · L -1 NAA inhibited the growth of white hairy roots and decreased the content of diosgenin per gram of dry matter.