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研究化学物质对癌细胞 DNA 或 RNA 作用形式来探讨癌发生的本质及治疗癌症的途径是十分有意义的。细胞的生物合成,如DNA 分子合成过程,可用放射性同位素标记的前体分子以放射自显影或液体闪烁计数技术示踪观察。胸腺嘧啶核苷(TdR)是DNA 分子的特殊前体,测定氘标记的胸腺嘧啶核苷(~3H—TdR)掺入到 DNA 分子中的放射性,能客观、灵敏地反映 DNA 合成的情况。本文通过测定掺入到小鼠腹水型肉瘤 S_(180)细胞的~3H—TdR 放射性,初步探讨先锋霉素Ⅱ对培养的癌细胞 DNA 合成的影响。
It is of great interest to investigate the nature of carcinogenesis and the ways to treat cancer by studying the form of the action of chemicals on DNA or RNA in cancer cells. Cell biosynthesis, such as DNA molecular synthesis, can be traced using radioisotope-labeled precursor molecules using either autoradiography or liquid scintillation counting techniques. Thymidine (TdR), a specific precursor of DNA, measures the radioactivity of deuterium labeled thymidine (~ 3H-TdR) into DNA molecules and objectively and sensitively reflects DNA synthesis. In this paper, the radioactivity of ~ 3H-TdR incorporated into mouse ascites sarcoma S_ (180) cells was measured to investigate the effect of cephamycin II on the DNA synthesis of cultured cancer cells.