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将黄曲霉素Ｂ１衍生成胺氧乙酸肟，然后分别同牛血清白蛋白和辣根过氧化物酶进行化学交联而制得结合物。前者用来免疫新西兰大白兔，后者用作免疫反应的示踪物。将所得抗体和示踪物在免疫反应中进行浓度优化，开发出了ＡＦＢ１酶联免疫分析直接竞争抑制法。用所建立的方法能够准确、快速地检测玉米和花生中黄曲霉素Ｂ１的含量。ＡＦＢ１在磷酸盐－吐温缓冲液中的最低检测限为０．４ｐｇ／ｍＬ；在玉米和花生中的最低检测限均为４０ｎｇ／ｋｇ。
Aflatoxin B1 was derivatized to oxamine oxime followed by chemical cross-linking with bovine serum albumin and horseradish peroxidase, respectively, to produce a conjugate. The former is used to immunize New Zealand white rabbits, the latter as a tracer of the immune response. The resulting antibodies and tracer were optimized for concentration in the immune response and the AFB1 enzyme-linked immunoassay direct competition inhibition assay was developed. The established method can accurately and quickly detect aflatoxin B1 in corn and peanut. The lowest detection limit of AFB1 in phosphate-Tween buffer was 0.4 pg / mL. The detection limit of both AFB1 in corn and peanut was 40 ng / kg.