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用套式聚合酶链反应(nPCR)及限制酶分析,病毒分离和特异性抗体测定检测孕妇外周血,脐带血及死胎组织巨细胞病毒(HCMV)。结果367名孕妇中,孕早、中、晚期HCMV阳性率分别为8.6％、1.6％及7.0％。nPCR检出率(4.9％)高于病毒分离(3.0％,P<0.025)。6份nPCR阳性母血中,3份配对脐血nPCR也阳性,母—脐传播率为50％(3/6)。28例死胎组织中,发现一死胎肺组织nPCR DNA阳性。提示:nPCR能提高诊断HCMV的特异性与敏感性,对孕妇及胎儿/新生儿HCMV感染的研究有重要意义。
Maternal peripheral blood, cord blood and stillbirth cytomegalovirus (HCMV) were detected by nested polymerase chain reaction (nPCR), restriction enzyme analysis, virus isolation and specific antibody assay. Results Among the 367 pregnant women, the positive rates of HCMV in the early, middle and late stages of pregnancy were 8.6%, 1.6% and 7.0% respectively. The detection rate of nPCR (4.9%) was higher than that of virus (3.0%, P <0.025). Of the 6 nPCR positive maternal blood, 3 matched cord blood nPCRs were also positive, with a mother-umbilical transmission rate of 50% (3/6). Twenty-eight cases of stillbirth were found to have positive nPCR DNA in the fetal lung tissue. Tip: nPCR can improve the diagnosis of HCMV specificity and sensitivity of pregnant women and fetal / neonatal HCMV infection of great significance.