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以含不同浓度 Zn~(2+)的实验液分别灌流豚鼠心室乳头肌标本,用玻璃微电极引导细胞内的电信号,输入紫金Ⅱ微机系统;对所记录图形用博士851程序进行采样、分析和存储,并将图形和分析结果打印。实验结果表明,低浓度 Zn~(2+)灌流可使心肌细胞复极化过程延长,特别是APD_(50)延长尤为明显。Zn 可使 Ca~(2+)内流受阻,对抗由于细胞内 Ca~(2+)超负荷所引起的心肌细胞损伤。随着 Zn~(2+)浓度的增大,Ca~(2+)内流严重受阻,心肌细胞不能维持必要的 Ca~(2+)内流使 APD 逐渐缩短,特别是 APD_(50)缩短更为显著。
Guinea pig ventricular papillary muscles were perfused with different concentrations of Zn ~ (2+) test solution, and the electrical signals of the cells were guided by glass microelectrodes and input into Zijin Ⅱ microcomputer system. The recorded patterns were sampled and analyzed by Dr. 851 program And store and print the graphs and analysis results. The experimental results showed that the perfusion of low concentrations of Zn2 + could prolong the repolarization of cardiomyocytes, especially the prolongation of APD_ (50). Zn can block Ca2 + influx, and resist cardiomyocyte injury caused by intracellular Ca2 + overload. As the concentration of Zn 2+ increased, the Ca 2+ influx was severely blocked, and the cardiomyocytes could not maintain the necessary Ca 2+ influx to shorten the APD, especially APD 50. More significant.