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采用ＲＰ－ＨＰＬＣ法测定益气活血注射液中黄芪甲苷的含量。用十八烷基硅烷键合相柱，乙腈－水（３１∶６９）为流动相，检测波长２０３ｎｍ。经试验，该法线性关系良好（ｒ＝０．９９９９），回收率为９７．５８％（ｎ＝６），ＲＳＤ为１．１９％。可作为益气活血注射液的含量测定方法。
Determination of Astragaloside Ⅳ in Yiqi Huoxue Injection by RP-HPLC. With octadecylsilane bonded phase, acetonitrile-water (31:69) as the mobile phase, detection wavelength of 203nm. The test showed good linear relationship (r = 0.9999), recovery of 97.58% (n = 6) and RSD of 1.19%. Can be used as the content of Qi and activating blood injection method.