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Objective:To evaluate the possible antifilarial effect of ethyl acetate extract of Vitex negundo(Verhenaceae)leaves against Selaria cervi filarial parasite in vitro.Methods:In vitro screening was done by the method of motility inhibition and NTT reduction assay with concentralions of0.03 to 1.00 mg/mL for 2 to 24 h incubation periods respectively,for possible antifilarial effect by comparing with control.Results:In motility assay,complete inhibition of motility was observed and in MTT reduction assay which gave>50%reduction for concentrations 0.20,0.50and 1.00 mg/mL at 10,6 and 2 h incubation periods respectively in a dose dependent manner(P<0.05).An antifilarial effect imparted by plant extract was found to be a function of their relative concentrations.Inhibitory concentration(IC_(50))for the plant extract was found to be 0.16mg/mL.Conclusions:The present study recorded significant antifilarial effect of Vitex negundo plant extract and contributed toward the development of database for novel drug candidates for lymphatic fllariasis.
Objective: To evaluate the possible antifilarial effect of ethyl acetate extract of Vitex negundo (Verhenaceae) leaves against Selaria cervi filarial parasite in vitro. Methods: In vitro screening was done by the method of motility inhibition and NTT reduction assay with concentrations of 0.03 to 1.00 mg / mL for 2 to 24 h incubation periods respectively, for possible antifilarial effect by comparing with control. Results: In motility assay, complete inhibition of motility was observed and in MTT reduction assay which gave> 50% reduction for concentrations 0.20, 0.50 and 1.00 mg / mL at 10,6 and 2 h incubation periods respectively in a dose dependent manner (P <0.05). An antifilarial effect imparted by plant extract was found to be a function of their relative concentrations. Inhibitory concentration (IC 50 )) for the plant extract was found to be 0.16 mg / mL. Conclusions: The present study recorded significant antifilarial effect of Vitex negundo plant extract and contributed toward the development of database f or novel drug candidates for lymphatic fllariasis.