在杂交瘤制备过程中,与细胞融合同等重要的,是维持最佳生长和抗体产生的饲养层细胞的作用。现已明确,不是饲养细胞本身,而是它们产生的淋巴因子能增强杂交瘤细胞生长和抗体产生。这些淋巴因子是同源性的;事实上,那些从人细胞衍生的因子似乎是相同的。例如,人单核细胞衍生的白细胞介素6(IL-6),原称为杂交瘤生长因子(HGF);从它的其他名称可反映其不同活性——B细胞刺激因子2(BSF-2)、肝细胞刺激因子(HSF)、β2干扰素(IF Equally important to cell fusion during hybridoma preparation is the role of feeder cells that maintain optimal growth and antibody production. It is now clear that instead of the feeder cells themselves, the lymphokines they produce can enhance hybridoma cell growth and antibody production. These lymphokines are homologous; in fact, those derived from human cells seem to be the same. For example, human monocyte-derived interleukin 6 (IL-6), formerly known as hybridoma growth factor (HGF); from its other names reflect its different activity - B cell stimulating factor 2 (BSF-2 ), Hepatocyte stimulating factor (HSF), beta 2 interferon (IF