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目的:研究右旋糖苷所致IgA肾病小鼠的肾组织蛋白表达变化和黄芪提取物(AEAR)对IgA肾病小鼠的作用。方法:给正常小鼠注射右旋糖苷建立IgA肾病小鼠模型。部分模型小鼠给予AEAR10g·kg-1·d-1作为AEAR治疗组。为探究AEAR的作用,运用双向电泳(2-DE)技术分别获得正常对照组、IgA肾病模型组、AEAR组小鼠的肾组织蛋白凝胶,银染后,图像用PDQUEST双维分析软件分析比较,得到相应图谱。结果:连续12周给予AEAR的IgA肾病小鼠血清尿素氮(BUN)、肌酐(Cr)和尿蛋白含量明显降低,且它们的肾组织蛋白图谱相应恢复接近正常鼠的图谱形态。与正常组比较,模型组有334个蛋白表达有显著性差异,其中142个蛋白表达增强2倍,61个蛋白表达增强5倍,121个蛋白表达减弱2倍;10个蛋白在模型组特异表达,在正常组不表达。以上334个差异蛋白中的50%个蛋白在给予AEAR治疗后,表达水平趋近于正常对照组水平;上述10个特异表达的蛋白经过AEAR干预后,有5个蛋白恢复至正常的不表达状态,4个蛋白表达明显减弱接近正常水平,只有1个蛋白表达上调甚至高于模型组对应蛋白的表达水平,分析可能与IgA肾病发病或AEAR药物作用密切相关。结论:AEAR不仅具有保护IgA肾病小鼠肾脏功能的作用,而且能调节小鼠肾脏组织蛋白表达,有助于发现AEAR作用于IgA肾病的靶蛋白。
Objective: To study the changes of renal tissue protein expression in dextran-induced IgA nephropathy mice and the effect of Astragalus extract (AEAR) on IgA nephropathy mice. Methods: Normal mice were injected dextran to establish IgA nephropathy mouse model. Some models of mice given AEAR10g · kg-1 · d-1 as AEAR treatment group. In order to explore the role of AEAR, renal tissue protein gel of normal control group, IgA nephropathy model group and AEAR group were obtained by two-dimensional electrophoresis (2-DE) technique. After silver staining, the images were analyzed by PDQUEST software , Get the corresponding map. Results: Serum urea nitrogen (BUN), creatinine (Cr) and urinary protein in IgA nephropathy mice that were given AEAR for 12 consecutive weeks were significantly lower than those in normal mice, and their renal histological patterns were restored to those of normal mice. Compared with the normal group, 334 protein expression in the model group was significantly different, of which 142 protein increased 2-fold, 61 protein increased 5-fold, 121 protein decreased 2-fold; 10 protein-specific expression in the model group , Not expressed in normal group. Fifty-three percent of the above 334 differentially expressed proteins were treated with AEAR, and the expression level approached that of the normal control group. After the above 10 proteins were specifically expressed, 5 proteins returned to the normal non-expressing state , 4 protein expression was significantly weakened to near normal level, only 1 protein was up-regulated or even higher than that of model group. The analysis may be closely related to the onset of IgA nephropathy or the effect of AEAR drug. CONCLUSION: AEAR not only protects the kidneys of mice with IgA nephropathy, but also regulates the protein expression of kidney in mice, which helps to find the target protein of AEAR in IgA nephropathy.