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目的:研究外源细胞分裂素6-苄氨基嘌呤(6-BA)处理对甘草中主要药用成分的影响,探究甘草中各化学成分之间的调控网络。方法:以两年生甘草Glycyrrhiza uralensis Fisch.植株为试验材料,分别于甘草生长期的两个不同阶段6月、7月喷施4种不同浓度(15、25、50、100 mg/L)的6-BA,利用HPLC检测7种化学成分(甘草酸、甘草苷、异甘草苷、甘草素、异甘草素、芹糖基甘草苷和芹糖基异甘草苷)的含量,分析外源刺激后甘草中7种成分含量的动态变化。结果:(1)6月份6-BA处理各组,7种成分含量的提高幅度最大值均远大于7月份处理组,其中,6月份100 mg/L的6-BA处理3个月后,其7种成分提高幅度分别比对照高出59.34%、71.14%、57.31%、16.36%、30.17%、80.26%、91.90%;(2)不同浓度处理组影响最大的成分为甘草酸;(3)6-BA处理有抵御自然生长条件下甘草中黄酮类含量降低的作用;(4)6-BA处理虽然对甘草中的化学成分含量有一定影响,但并不改变其化学成分间组成变化。结论:外源6-BA处理可以同时提高甘草中甘草酸含量以及黄酮类物质的积累。
OBJECTIVE: To study the effect of exogenous cytokinin 6-benzylaminopurine (6-BA) on the main medicinal components in Glycyrrhiza uralensis and to explore the regulation network of the chemical constituents in Glycyrrhiza uralensis. Methods: Two biennial Glycyrrhiza uralensis Fisch. Plants were used as test materials. Four different concentrations (15, 25, 50 and 100 mg / L) of 6 -BA. The content of seven chemical components (glycyrrhizin, glycyrrhizin, isoliquiritigenin, glycyrrhizin, isoliquiritigenin, celery glycyrrhizin and glucosyl-isoliquiritigenin) were detected by HPLC. In seven kinds of ingredients in the dynamic changes. Results: (1) In 6-BA treatment in June, the maximal increment of the contents of 7 components were much higher than that in July. Among them, 6-BA treated with 100 mg / L in June was (2) The most influential components of the seven treatments were glycyrrhizic acid; (3) the content of the seven components increased by 59.34%, 71.14%, 57.31%, 16.36%, 30.17%, 80.26%, 91.90% -BA treatment had the effect of reducing the flavonoids content of licorice under the condition of natural growth; (4) 6-BA treatment did not change the composition of the chemical composition of licorice, although it had some influence on the chemical composition. Conclusion: Exogenous 6-BA can increase the content of glycyrrhizic acid and the accumulation of flavonoids in licorice.