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目的建立采用双波长HPLC同时测定青阳参中青阳参苷元和告达庭含量的方法。方法采用高效液相色谱法,ZORBAX SB-C18(4.6 mm×150 mm,5μm)色谱柱,柱温40℃,流动相为乙腈-水,梯度洗脱,流速为1.0 mL·min·1,检测波长为220,260 nm。结果青阳参苷元和告达庭均在2~64μg·mL·1内呈良好的线性关系,r均为1;平均回收率为100.1%和97.9%,RSD值分别为1.34%和1.37%(n=6)。结论该方法快捷简单,精密可靠,重复性好,可为青阳参及其制剂的质量控制提供参考。
Objective To establish a dual-wavelength HPLC simultaneous determination of Qingyang ginseng glycosides and gynostemtin content method. Methods High performance liquid chromatography (HPLC) was performed on a ZORBAX SB-C18 column (4.6 mm × 150 mm, 5 μm) with a column temperature of 40 ° C. The mobile phase consisted of acetonitrile-water with a gradient of 1.0 mL · min · 1. The wavelength is 220,260 nm. Results The results showed that there was a good linear relationship between yammethrin and procampin in the range of 2 ~ 64μg · mL · 1, r was 1 and the average recoveries were 100.1% and 97.9% with RSDs of 1.34% and 1.37% (n = 6). Conclusion The method is fast, simple, accurate, reliable and reproducible. It can provide references for the quality control of Qingyangshen and its preparations.