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目的通过对临床常用化疗药物５－氟尿嘧啶（５－ＦＵ）、丝裂霉毒（ＭＭＣ）、卡铂（ＣＢＰ）、阿霉素（ＡＤＭ）和表阿霉素（Ｅ－ＡＤＭ）相互配伍诱导人胰腺癌细胞株细胞凋亡的观察，探讨化疗药物抑制胰腺癌细胞生长的作用机制，为临床制定合理的化疗方案提供理论依据。方法不同配伍的化疗药物作用于胰腺癌细胞后，以倒置显微镜和荧光显微镜观察其形态变化；以流式细胞仪检测细胞ＤＮＡ含量的变化，计算出凋亡细胞的比例；提取细胞ＤＮＡ，进行凝胶电泳确定细胞凋亡的发生。结果联合化疗可以诱导胰腺癌细胞凋亡，与引起细胞坏死相比，诱导肿瘤细胞凋亡的化疗药物剂量小、作用时间短；随作用时间的延长和药物剂量的增加，凋亡细胞的比例也增加；不同配伍的化疗药物诱导不同胰腺癌细胞株细胞发生凋亡的情况是不完全相同的。结论诱导肿瘤细胞凋亡是化疗药物抑制胰腺癌细胞生长的主要机制，能否诱导胰腺癌细胞发生凋亡可作为判断不同配伍的化疗药物抑制胰腺癌细胞生长能力的重要指标之一。
OBJECTIVE To study the effects of 5-fluorouracil (5-FU), mitomycin C (MMC), carboplatin (CBP), doxorubicin (ADM) and epirubicin (E-ADM) Pancreatic cancer cell apoptosis observation, to explore the mechanism of chemotherapy drugs to inhibit the growth of pancreatic cancer cells, to provide a theoretical basis for the development of a reasonable chemotherapy regimen. Methods Different combinations of chemotherapeutic drugs on pancreatic cancer cells were observed by inverted microscope and fluorescence microscope morphological changes; changes in cellular DNA content was detected by flow cytometry to calculate the proportion of apoptotic cells; cell DNA was extracted for coagulation Gel electrophoresis to determine the occurrence of apoptosis. Results Combined chemotherapy can induce apoptosis of pancreatic cancer cells. Compared with the induction of apoptosis, the chemotherapeutic agents inducing apoptosis of tumor cells are small and the action time is short. With the prolongation of the action time and the increase of the dosage, the proportion of apoptotic cells Increase; different compatibility of chemotherapy drugs to induce apoptosis in different pancreatic cancer cell lines is not exactly the same situation. Conclusion Induction of apoptosis in tumor cells is the main mechanism by which chemotherapeutic drugs inhibit the growth of pancreatic cancer cells. Whether pancreatic cancer cells can induce apoptosis can be used as an important index to judge the ability of chemotherapeutics to inhibit the growth of pancreatic cancer cells.