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目的:探讨骨髓间充质干细胞(MSCs)移植对裸鼠急性肾小管坏死(ATN)的修复作用,为MSCs移植治疗ATN提供实验依据。方法:5周龄裸鼠随机分为健康对照组、ATN模型组、MSCs移植治疗组。ATN模型采用50%甘油肌内注射诱导,MSCs移植采用经肾动脉注射途径向肾脏内移植增强型绿色荧光蛋白(EGFP)标记的MSCs。实验期间观察饮食、活动等变化,检测血肌酐(Scr)水平,H-E染色观察肾组织病理学变化,荧光显微镜观察EGFP标记的阳性细胞在裸鼠肾脏的分布及数量。结果:ATN模型组裸鼠肾脏肾小管上皮细胞变性、局部坏死,病变于肾皮质或皮髓交界处明显,而肾小球未见明显异常。MSCs移植治疗组的肾小管上皮细胞无混浊肿胀和细胞核固缩溶解,间质无明显的充血和水肿。MSCs细胞移植后第14天的受体鼠肾小管中EGFP阳性细胞明显增多。结论:肾动脉注射途径移植的MSCs能定位于肾小管上皮,可促进ATN中损伤的肾小管上皮细胞的修复。
Objective: To investigate the repair effect of bone marrow mesenchymal stem cells (MSCs) transplantation on acute tubular necrosis (ATN) in nude mice and provide experimental evidence for the transplantation of ATN. Methods: 5-week-old nude mice were randomly divided into healthy control group, ATN model group and MSCs transplantation group. The ATN model was induced by intramuscular injection of 50% glycerol. MSCs were transplanted into the kidney with enhanced green fluorescent protein (EGFP) labeled MSCs via renal artery injection. The changes of diet, activity and so on were observed during the experiment. The level of Scr was detected. The pathological changes of renal tissue were observed by H-E staining. The distribution and number of EGFP-labeled positive cells in the kidney of nude mice were observed by fluorescence microscopy. Results: The renal tubular epithelial cells of ATN model group were degenerated and necrotic, and the pathological changes were obvious at the border of renal cortex or dermis, but no obvious abnormality was observed in glomerulus. The renal tubular epithelial cells in MSCs transplantation group had no swelling and pyknosis and no obvious congestion and edema in the interstitium. On the 14th day after MSCs transplantation, the number of EGFP positive cells in the tubular cells of the recipient rats increased significantly. CONCLUSIONS: MSCs transplanted via the renal artery can be localized in the renal tubular epithelium and promote the repair of injured renal tubular epithelial cells in ATN.