论文部分内容阅读
目的建立用于测定淫羊藿中淫羊藿苷含量的反相高效液相色谱法。方法采用ZORBAX Ecilpse XDB-C_(18)(150 mm×4.6 mm,5μm)色谱柱;以水-乙腈(74:26,V/V)为流动相;检测波长270 nm;流速1.0 mL·min~(-1),柱温25℃。结果淫羊藿苷质量浓度在5~50 mg·L~(-1)内与其峰面积之间线性关系良好(r=0.999 9),平均加样回收率为100.32%,RSD为0.44%。结论本方法操作简便、快速,结果准确,可用于淫羊藿中淫羊藿苷的含量测定。
Objective To establish a reversed-phase high performance liquid chromatography method for the determination of icariin in Epimedium. Methods ZORBAX Ecilpse XDB-C_(18)(150 mm×4.6 mm,5μm) column was used. The mobile phase was water-acetonitrile (74:26,V/V). The detection wavelength was 270 nm. The flow rate was 1.0 mL·min~ (-1), column temperature 25°C. Results The linear relationship between icariin mass concentration and its peak area was good in the range of 5-50 mg·L -1 (r=0.999 9). The average recovery was 100.32%, and the RSD was 0.44%. Conclusion The method is simple, rapid and accurate. It can be used for the determination of icariin in Epimedium.