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目的 对胃粘膜相关淋巴组织( MALT) 淋巴瘤浸润子宫平滑肌瘤病理形态进行定量测定研究。方法 对瘤组织采用常规石蜡切片、HE 染色及ABC 法免疫组化染色, 并运用HPIAS- 1000 彩色病理图文分析系统对肿瘤细胞核的形态进行定量测定。结果 胃粘膜、粘膜下层、肌层及平滑肌瘤内均可见弥漫多量密集成片的中心细胞样瘤细胞浸润, 在胃粘膜内并可见淋巴上皮病变。定量测定的结果表明胃、淋巴结及子宫肌瘤内淋巴瘤细胞细胞核15 项形态参数与对照组间除一项参数外( 胃淋巴瘤细胞与对照组间细胞核的椭圆度) , 所有的指标均有高度显著性差异( P< 0-001) 。结论 MALT 淋巴瘤细胞与正常淋巴细胞形态学参数之间确实具有差异, 定量测定的方法要比用肉眼在光镜下直接观察更为敏感, 其对于MALT 淋巴瘤疑难病例的诊断提供了新的诊断方法, 可能具有十分重要的意义
Objective To investigate the pathological features of gastric mucosa-associated lymphoid tissue (MALT) lymphoma infiltrating uterine leiomyoma. Methods The paraffin sections, HE staining and ABC immunohistochemical staining were performed on the tumor tissues. The morphology of tumor nuclei was quantitatively determined using the HPIAS-1000 color pathology analysis system. Results In the gastric mucosa, submucosa, myometrium and leiomyomas, a large number of densely integrated central cell-like tumor cells infiltrated. Gastric epithelial lesions were observed in the gastric mucosa. Quantitative measurement results showed that the morphological parameters of the nucleus of lymphoma cells in the stomach, lymph nodes, and uterine fibroids were all but one of the parameters (the ellipticity of the nucleus between the gastric lymphoma cells and the control group). Highly significant difference (P<0-001). Conclusions There is indeed a difference between morphological parameters of MALT lymphoma cells and normal lymphocytes. Quantitative determination methods are more sensitive than direct observation with the naked eye under light microscope. It provides a new diagnosis for the diagnosis of difficult cases of MALT lymphoma. Method may have very important significance