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本文通过原位杂交技术检测鼻咽活检组织不同病理改变时EBV—DNA的表现,以探讨EB病毒在鼻咽癌发生过程中的可能作用。结果显示:(1)本实验所有鼻咽低分化癌癌组织均出现EBV—DNA片段,且绝大部分病例的阳性细胞数及细胞阳性强度均在中等以上;(2)无论是鼻咽癌或慢性鼻咽炎病例,所见到的中-重度异型改变上皮或淋巴组织,均有数量不等的细胞存在阳性强度不同的EBV-DNA片段,粘膜下小涎腺内亦常见到一定数量的EBV-DNA片段。结果提示:(1)EB病毒与鼻咽低分化癌关系密切。EBV作为致鼻咽癌病因多因素中的一员是有可能的;(2)患者血清中EB病毒相关抗体,尤其是IgA抗体阳性率和滴度的升高,可能与鼻咽局部EBV感染及/或EBV激活有关;(3)在异型改变上皮中有中等或以上阳性强度EBV-DNA片段的检出,将有可能作为推测鼻咽癌癌前病变的有效指标。
In this paper, the in situ hybridization was used to detect the expression of EBV-DNA in different pathological changes of nasopharyngeal biopsy to explore the possible role of EBV in the development of nasopharyngeal carcinoma. The results showed that: (1) EBV-DNA fragments were present in all nasopharyngeal poorly differentiated cancer tissues in this experiment, and the majority of the cases had positive cell numbers and positive cell strengths; (2) whether nasopharyngeal carcinoma or In the cases of chronic nasopharyngitis, moderate-heavy dysplasia was observed in epithelial or lymphoid tissues, and there were EBV-DNA fragments with different positive intensities in different numbers of cells, and a certain number of EBVs were also found in the submucosal small salivary glands. DNA fragment. The results suggest that: (1) EB virus is closely related to poorly differentiated nasopharyngeal carcinoma. It is possible that EBV may be a member of many factors that cause nasopharynx cancer; (2) Epstein-Barr virus-associated antibody, especially IgA antibody positive rate and titer increase in serum, may be related to local EBV infection in nasopharyngeal (3) The detection of EBV-DNA fragments with moderate or more positive intensities in the heterotypic changes of the epithelium may be an effective indicator of the precancerous lesions of NPC.