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目的：以连二亚硫酸钠造成原代培养的大鼠胎鼠脑皮质神经元缺氧损伤，观察神经生长因子（ＮＧＦ）对缺氧损伤神经元的影响．方法：测定神经元生存力及细胞外液乳酸脱氢酶（ＬＤＨ）的活性来分析ＮＧＦ的作用，脑皮质细胞匀浆用于测定丙二醛（ＭＤＡ）含量及超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＳＨＰｘ）活性．结果：缺氧后，ＬＤＨ释放及细胞生存力降低．ＮＧＦ（１－１００μｇ·Ｌ－１）浓度依赖地减少ＬＤＨ的释放及ＭＤＡ的生成，ＮＧＦ１００μｇ·Ｌ－１显著提高细胞生存力及提高ＳＯＤ和ＧＳＨＰｘ活性２７倍．结论：ＮＧＦ通过减少脂质过氧化物生成及提高ＳＯＤ和ＧＳＨＰｘ活性来保护大脑皮质细胞抗缺氧损伤
OBJECTIVE: To hypoxia injury of primary cultured rat fetal rat cortical neurons with sodium hydrosulfite and to observe the effect of nerve growth factor (NGF) on neurons under hypoxia. Methods: The effect of NGF on neuronal viability and activity of extracellular fluid lactate dehydrogenase (LDH) was assayed. The content of malondialdehyde (MDA) and superoxide dismutase (SOD) And glutathione peroxidase (GSH Px) activity. Results: After hypoxia, LDH release and cell viability decreased. NGF (1-100μg · L-1) reduced LDH release and MDA production in a concentration-dependent manner. NGF (100μg · L-1) significantly increased cell viability and increased activity of SOD and GSH-Px by 2.7 times. Conclusion: NGF protects cerebral cortex cells against hypoxia injury by decreasing the production of lipid peroxides and increasing the activities of SOD and GSH-Px