In this study, two novel dual-switch fluorescent chemosensors based on rhodamine-peryleneiimide have been designed and synthesized. The dual-switching behaviors of the sensors were based on the structural transformations of rhodamine and an intramolecular photoinduced electron transfer(PET)process from rhodamine to perylenediimide. These probes exhibited excellent sensitivity to protons with enhanced fluorescence emission from 500 nm to 580 nm. The fluorescence changes of probes were reversible within a wide range of p H values from 2.0 to 11.0. Moreover, the sensors exhibited high selectivity, short response time, and long lifetime toward protons. The possible mechanism was investigated by the DFT calculation and ~1H NMR. According to the experiment of confocal laser scanning microscopy, these probes could be used to detect the acidic p H variations in living cells. In this study, two dual-switch behaviors of the sensors were based on the structural transformations of rhodamine and an intramolecular photoinduced electron transfer (PET) process from rhodamine These probes exhibit excellent sensitivity to protons with enhanced fluorescence emission from 500 nm to 580 nm. The fluorescence changes of probes were reversible within a wide range of p H values ​​from 2.0 to 11.0. response time, and long lifetime toward protons. The possible mechanism was investigated by the DFT calculation and ~ 1H NMR. According to the experiment of confocal laser scanning microscopy, these probes could be used to detect the acidic p H variations in living cells.