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目的:通过研究急性丙型肝炎病毒(HCV)感染患者外周血中FOXP3的表达,初步探讨其在急性丙型肝炎发病机制中的临床意义。方法:选择急性丙型肝炎患者与健康人各21例,用流式细胞仪检测外周血CD4+CD25+T细胞的数量;ELISA试剂盒检测患者和正常对照组外周血培养上清中Th2型细胞因子(IL-10)和Th1型细胞因子(IL-2)的表达;RT-PCR检测FOXP3的mRNA表达。结果:在急性HCV患者外周血中CD4+CD25+T细胞约占CD4+T细胞(28.2±2.1)%,显著高于正常人外周血中CD4+CD25+T细胞(2.7±0.7)%(P=0.032);急性HCV组外周血CD4+CD25+T细胞高表达FOXP3;急性HCV组外周血单个核细胞(PBMC)培养上清主要分泌IL-10,而IL-2分泌无显著变化。结论:急性HCV感染者Th1免疫抑制可能与外周血CD4+CD25+T细胞高表达FOXP3有关。
Objective: To investigate the clinical significance of FOXP3 expression in peripheral blood of patients with acute hepatitis C virus (HCV) infection and its clinical significance in the pathogenesis of acute hepatitis C infection. Methods: Twenty-one patients with acute hepatitis C and healthy individuals were selected. The number of CD4 + CD25 + T cells in peripheral blood was detected by flow cytometry. Th2 cells in peripheral blood culture supernatant of patients and normal controls were detected by ELISA kit (IL-10) and Th1 type cytokines (IL-2) were detected by RT-PCR. The mRNA expression of FOXP3 was detected by RT-PCR. Results: The percentage of CD4 + CD25 + T cells in peripheral blood of patients with acute HCV was (28.2 ± 2.1)%, which was significantly higher than that of CD4 + CD25 + T cells (2.7 ± 0.7)% (P = 0.032). Peripheral blood CD4 + CD25 + T cells were highly expressed FOXP3 in acute HCV group. IL-10 secretion was mainly secreted by peripheral blood mononuclear cells (PBMC) in acute HCV group, but there was no significant change in IL-2 secretion. Conclusion: Th1 immunosuppression in acute HCV infection may be related to the overexpression of FOXP3 in peripheral blood CD4 + CD25 + T cells.