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目的:比较长叶胡颓子地上部位与地下部位体内外抗氧化作用,进行长叶胡颓子地上部位开发利用价值及抗动脉粥样硬化(AS)的可行性评价。方法:测定长叶胡颓子各部位在低、中、高质量浓度(5,10,20 g·L-1)对羟自由基(·OH)和超氧阴离子自由基(O-·2)的清除率;观察各部位在低、中、高质量浓度(5,10,20 g·L-1)对铜离子诱导的健康人静脉血低密度脂蛋白(LDL)氧化的影响,测定LDL氧化延迟时间(lag time)和达最大氧化速率时间(Tmax),硫代巴比妥酸(TBA)比色法测定反应液中丙二醛(MDA)含量;选用KM小鼠颈背部皮下注射5%D-半乳糖造模8周后,灌胃给予低、中、高剂量(2,5,10 g·kg-1)长叶胡颓子各部位提取液,14 d后眼眶取血,分离血清,观察长叶胡颓子各部位对血清总抗氧化能力(TAC)、谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)等抗氧化酶活性、丙二醛(MDA)含量的影响。结果:长叶胡颓子地上部位低、中、高各剂量组对O-·2清除率分别为(58.26±6.14)%,(68.21±3.47)%和(74.67±4.10)%,对·OH清除率分别为(48.24±6.24)%,(78.21±7.24)%和(90.17±6.58)%,二者对自由基的清除率均高于地下部位各剂量组,同时长叶胡颓子地上部位和地下部位均能显著增加LDL的Lag time和Tmax,其结果与其减少铜离子诱导的MDA生成作用相一致;血清TAC,SOD,GPX活性均升高,能有效降低小鼠血清MDA含量,其地上部位活性升高显著。结论:长叶胡颓子各部位具有良好的体内外抗氧化活性,能明显提高血清及LDL抗氧化能力,同时长叶胡颓子地上部位抗氧化活性显著优于地下部位。
OBJECTIVE: To compare the antioxidation effect of Elaeagnus bockii and its underground parts both in vitro and in vivo, and to evaluate the feasibility of anti-atherosclerosis (AS) development and utilization of Elaeagnus bockii. Methods: The effects of low, medium and high concentrations (5, 10, 20 g · L -1) of hydroxyl radicals (· OH) and superoxide anion radical (O- · 2) (5,10,20 g · L-1) on the oxidation of LDL induced by Cu2 + in healthy volunteers. The effects of low, medium and high concentrations (5, 10, 20 g · L-1) The lag time and the maximum oxidative rate time (Tmax) were determined. The content of malondialdehyde (MDA) in the reaction solution was determined by thiobarbituric acid (TBA) colorimetric assay. KM mice were injected subcutaneously with 5% D-galactose model 8 weeks later, low, medium and high dose (2, 5, 10 g · kg-1) leaves of Elaeagnus pubescens extracts of various parts, 14 days after orbital bleeding, serum To observe the effects of different parts of Elaeagnus angustifolia on the activities of antioxidant enzymes (TAC), glutathione peroxidase (GPX) and superoxide dismutase (SOD), malondialdehyde (MDA) ) Content of the impact. Results: The clearance rates of O- · 2 in the low, middle and high doses of Elaeagnus bockii were (58.26 ± 6.14)%, (68.21 ± 3.47)% and (74.67 ± 4.10)%, respectively. (48.24 ± 6.24)%, (78.21 ± 7.24)% and (90.17 ± 6.58)%, respectively. The clearance rates of free radicals in the two groups were all higher than those in the underground, And underground parts significantly increased the LDL Lag time and Tmax, the results consistent with its role in reducing the copper ion-induced MDA generation consistent; serum TAC, SOD, GPX activity were increased, can effectively reduce the level of serum MDA in mice, the ground Site activity was significantly increased. Conclusion: Elaeagnus bockii has good antioxidant activity in vitro and in vivo, and can significantly improve the antioxidant capacity of serum and LDL. At the same time, the antioxidant activity of Elaeagnus bockii is significantly superior to the underground parts.