目的:探讨不同年龄儿童SIRT1的表达水平与幽门螺旋杆菌(H. pylori)感染相关性.方法:收集3-25天、6个月-2岁、3-6岁幼儿的血清及粪便,采用ELISA检测血清及粪便中SIRT1表达水平.建立小鼠H. pylori感染模型,采用ELISA检测不同分组小鼠SIRT1、ROS、SOD2表达水平.结果:儿童血清(F=195.97、P＜0.01)及粪便(F=321.30、P＜0.01)中SIRT1表达水平均随年龄增大显著降低.H. pylori感染阳性儿童血清(t=16.13、P＜0.01)及粪便(“,”Objective:To investigate the correlation both SIRT1 expression levels and different ages of children with Helicobacter pylori(H. pylori)infection. Methods:Collected serum and feces of children aged 3-25 days,6 months-2 years and 3-6 years,SIRT1 expression levels were detected by ELISA assay. Establish a mouse H. pylori infection model, and use ELISA to detect the expression levels of SIRT1, ROS,and SOD2 in different groups of mice.Results:The expression level of SIRT1 in children\'\'s serum (F=195.97,P＜0.01)and feces(F=321.30,P＜0.01)decreased significantly with age.The levels of SIRT1 in children with positive H.pylori infection (t=16.13, P＜0.01) and stool(t=14.57, P＜0.01)were significantly lower than those in children with negative H. pylori infection. The expression levels of SIRT1 and SOD2 in the stomach tissues of mice infected with H.pylori were significantly lower than those in the uninfected group (P＜0.05), while the expression levels of ROS increased(P＜0.001).Conclusion:The expression level of SIRT1 in children\'\'s serum and feces is negatively correlated with children\'\'s age.H.pylori can induce mitochondrial dysfunction by down-regulating the expression of SIRT1.