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目的:改变实验用菌种,测定硫酸庆大霉素的含量,使结果更加准确可靠。方法:采用抗生素微生物检定法中的管碟法,改用枯草芽孢杆菌为实验菌种,测定硫酸庆大霉素的含量。结果:硫酸庆大霉素的线性范围为1～12μg·ml~(-1)(r=0.992 7),平均回收率为:96.6%;样品测定中用枯草芽孢杆菌所得结果的可信限明显低于用短小芽孢杆菌所得结果的可信限,结果更加准确、可靠。
Objective: To change the experimental bacteria and determine the content of gentamicin sulfate to make the result more accurate and reliable. Methods: Using the method of plate and dish in the method of antibiotic microbe test, Bacillus subtilis was used as experimental strain to determine the content of gentamicin sulfate. Results: The linear range of gentamicin sulfate was 1 ~ 12μg · ml ~ (-1) (r = 0.992 7) with an average recovery of 96.6%. The confidence limits of the results obtained with the sample from Bacillus subtilis were obvious Lower than that of the result obtained with B. pumilus, the result is more accurate and reliable.