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目的了解河北省病毒性腹泻监测哨点医院5岁以下所有腹泻住院患儿轮状病毒的流行特征,为轮状病毒感染性腹泻的防治提供科学依据。方法收集哨点医院2010～2012年5岁以下所有腹泻住院患儿发病3日内粪便标本。使用ELISA法检测A组人类轮状病毒抗原,采用巢式逆转录-聚合酶链反应对轮状病毒抗原阳性标本进行分型鉴定。结果 1 155份粪便标本中514份标本检测出轮状病毒抗原,阳性率为44.50%,12～17月龄段患儿轮状病毒抗原阳性率最高,达到58.54%。轮状病毒感染有明显的季节性,主要集中在11月到次年的2月,其中11～12月份阳性率最高,达到63.15%。血清型G型中主要流行血清型为G9型,占42.22%;P型中P[8]型最多,占66.34%,G/P组合型主要型别为G9P[8(]占35.21%)。结论轮状病毒是河北省5岁以下腹泻住院患儿感染的主要病原体,以G9P[8]为主要优势株。
Objective To understand the epidemiological characteristics of rotavirus in all children with diarrhea under 5 years old in sentinel hospital of viral diarrhea monitoring in Hebei province and provide scientific basis for the prevention and treatment of rotavirus infectious diarrhea. Methods The sentinel hospitals collected all the stool specimens within 3 days after the onset of infantile diarrhea in children who were under 5 years of age from 2010 to 2012. A group of human rotavirus antigen was detected by ELISA, and rotavirus antigen positive samples were identified by nested reverse transcription-polymerase chain reaction. Results The positive rate of rotavirus antigen in 514 samples from 1 155 stool specimens was 44.5%. The positive rate of rotavirus antigen in children aged 12-17 was 58.54%. Rotavirus infection has obvious seasonal, mainly in November to February of the following year, of which 11 to December the highest positive rate of 63.15%. Serotype G major epidemic serotype G9, accounting for 42.22%; P type P [8] type, accounting for 66.34%, G / P combination of the main type of G9P [8 (35.21%). Conclusions Rotavirus is the main pathogen in hospitalized children with diarrhea under 5 years old in Hebei province. G9P [8] is the predominant strain.