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目的 :观察抗卵巢癌药物顺铂、紫杉醇和阿霉素对卵巢癌细胞系 OVCAR- 3体外生长和生存的影响并分析由它们所致的细胞死亡性质。方法 :采用细胞形态学观察、细胞动力学检测及 DNA片段化分析等细胞和分子生物学方法 ,研究化疗药物对卵巢癌细胞的凋亡诱导作用。结果 :上述药物在抑制 OVCAR- 3细胞生长的同时可不同程度地诱导细胞凋亡。其中 ,紫杉醇诱导细胞凋亡的能力最强 ;较低剂量紫杉醇 (10 - 8M)和顺铂 (2 μg/ ml)配伍使用的抗癌效果优于单纯顺铂 (5 μg/ m l)或顺铂 (2 μg/ ml) +阿霉素 (0 .5 μg/ ml)。结论 :1)化疗药的抑癌作用主要是通过诱导卵巢癌细胞凋亡来实现 ;2 )抗癌药诱导细胞凋亡的能力与其生长抑制效应成正比 ;3)对靶细胞凋亡的诱导能力可作为筛选抗卵巢癌敏感化疗药物以及最佳配伍剂量的客观指标。
OBJECTIVE: To observe the effects of anti-ovarian cancer drugs cisplatin, paclitaxel and doxorubicin on the growth and survival of ovarian cancer cell line OVCAR-3 in vitro and to analyze the cell death properties caused by them. Methods: Cell and molecular biology methods such as cell morphological observation, cell kinetic assay and DNA fragmentation analysis were used to study the apoptosis induction effect of chemotherapeutics on ovarian cancer cells. Results: The above drugs can induce apoptosis of OVCAR-3 cells to varying degrees while inhibiting the growth of OVCAR-3 cells. Among them, paclitaxel had the strongest ability of inducing apoptosis; the lower anticoagulant effect of paclitaxel (10 - 8M) and cisplatin (2 μg / ml) was better than cisplatin (5 μg / ml) (2 μg / ml) + doxorubicin (0.5 μg / ml). Conclusions: 1) The anti-tumor effect of chemotherapeutic drugs is mainly through the induction of apoptosis in ovarian cancer cells; 2) the ability of anti-cancer drugs to induce apoptosis is directly proportional to their growth inhibitory effect; 3) the induction of target cells apoptosis Can be used as screening anti-ovarian cancer sensitive chemotherapeutic drugs and the best compatibility dose objective indicators.