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目的 研究利多卡因对兔内毒素性急性肺损伤的预防作用。方法 雄性健康新西兰兔 2 4只 ,分 3组 :空白组 (S -S ,输注生理盐水 ) ,内毒素组 (L -S ,输注内毒素后输注生理盐水 ) ,预防组 (Ld2 -L ,输注内毒素前 10min输注利多卡因负荷量 2mg·kg- 1 ,然后 2mg·kg- 1 ·h- 1 ) ,控制呼吸 ,吸氧浓度 95 % ,输注内毒素后 6h杀兔。观察氧合指数 (PaO2 FiO2 )、肺动态顺应性 (Cdyn)、支气管肺泡灌洗液 (BALF)白蛋白含量、肺湿 干重比值、肺组织超氧化物歧化酶 (SOD)、丙二醛 (MDA)和髓过氧化物酶 (MPO)以及肺形态学变化。结果 内毒素组PaO2 FiO2 、Cdyn 和肺组织SOD活性明显下降 ;BALF白蛋白含量、肺湿 干重比值和肺组织MDA和MPO含量明显上升 ;肺泡结构严重毁坏 ,肺泡间隔增宽 ,大量炎症细胞浸润。利多卡因预防可明显减轻这些变化。结论 利多卡因对兔内毒素性急性肺损伤有明显的预防作用
Objective To study the preventive effect of lidocaine on endotoxin-induced acute lung injury in rabbits. Methods Twenty - four male New Zealand rabbits were randomly divided into 3 groups: control group (S - S, infusion of normal saline), LPS group (L - S, saline infusion after endotoxin infusion) L, infusion of lidocaine 10min before infusion of lidocaine load 2mg · kg-1, then 2mg · kg-1 · h-1), control of breathing, oxygen concentration 95%, 6h after infusion of endotoxin to kill rabbits . PaO2 FiO2, Cdyn, albumin in bronchoalveolar lavage fluid (BALF), lung wet / dry weight ratio, lung tissue superoxide dismutase (SOD), malondialdehyde MDA) and myeloperoxidase (MPO) and lung morphological changes. Results The levels of PaO2 FiO2, Cdyn and SOD in lung tissue were significantly decreased in endotoxin group. The content of albumin in BALF and the ratio of wet to dry weight of lung and the content of MDA and MPO in lung tissue were significantly increased. The alveolar structure was severely damaged, the alveolar septum was broadened and a large number of inflammatory cells infiltrated . Lidocaine prevention can significantly reduce these changes. Conclusion Lidocaine has a significant preventive effect on endotoxin-induced acute lung injury in rabbits