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选用成年雄性Ｗｉｓｔａｒ大鼠，由平原（海拔５ｍ，上海）直接运送到高原（２２６１ｍ，西宁和３４６０ｍ，天峻）。以放射免疫分折法研究了大鼠在高原２４ｈ．急性缺氧期和３０ｄ慢性缺氧期中枢脑内β－内啡肽样免疫活性物质（β－ＥＰ）的含量变化。结果表明：１．大鼠在两个不同海拔（２２６１ｍ，３４６０ｍ）环境２４ｈ急性缺氧期与平原对照组相比，脑垂体内β－ＥＰ含量降低非常显著（Ｐ＜０．０１），纹状体、丘脑、下丘脑、桥－延脑、海马内β－ＥＰ含量均增加非常显著（Ｐ＜０．０１）。２．大鼠在３４６０ｍ高原３０ｄ慢性习服期，垂体及各脑区内β－ＥＰ的含量变化呈时相性：即１—３ｄ均呈进行性增加（Ｐ＜０．０１）：３－１５ｄ呈持续性减少，除中脑、纹状体、丘脑外，均为Ｐ＜０．０１。１５－３０ｄ垂体、丘脑、皮层、纹状体内β－ＥＰ含量仍持续减少（毒体、皮层Ｐ＜０．０１），桥－延脑、下丘脑、海马趋于回升（Ｐ＜０．０１），中脑亦趋于回升（Ｐ＞０．０５）。脑内β－ＥＰ的这种变化可能具有十分重要的生物学意义。
Adult male Wistar rats were selected and transported straight to the plateau (2261m, Xining and 3460m, Tianjun) from the plain (elevation 5m, Shanghai). Rats were exposed to high altitude for 24 hours by radioimmunoassay. Changes of content of β-endorphin-like immunoreactive substance (β-EP) in brain during acute hypoxia and 30-day chronic hypoxia. The result shows: 1. Compared with control group, the level of β-EP in the pituitary gland decreased significantly at 2 h (2261 m, 3460 m) in acute hypoxia stage in rats at two different elevations (P <0.01) The content of β-EP in thalamus, bridge - medulla oblongata and hippocampus increased significantly (P <0.01). 2. The rats in the 3460m plateau 30d chronic obsession, pituitary and brain regions of β-EP content showed a time-phase: namely, 1-3d showed a progressive increase (P <0.01): 3-15d was persistent (P <0.01.15-30d). The content of β-EP in pituitary, thalamus, cortex and striatum continued to decrease (except the midbrain, striatum and thalamus) (P <0.01 (P <0.01). The midbrain also tended to rebound (P> 0.05). This change in brain beta-EP may have very important biological implications.