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目的 观察APP17肽 (App17p)对D 半乳糖 (D gal)脑老化模型小鼠海马超微结构的影响。方法 用D gal制造小鼠脑老化模型 ,并皮下注射APP17p ,8wk后取小鼠海马CA1区进行电镜观察。结果 ①D gal组小鼠海马神经元轴索微管结构出现明显溶解 ;线粒体数量多于APP17p +D组 ,但差异无显著性 ;线粒体肿胀并有空泡变性 ,电子密度增高 ;核糖体及粗面内质网明显减少 ;神经毡中突触明显减少 ,与APP17p +D组相比 (P <0 0 5 ) ;海马神经元的结构未发现异常变化。②APP17p +D组小鼠海马神经元轴索中微管结构正常 ,微丝清晰 ;线粒体膜及结构正常 ,线粒体脊清晰 ;粗面内质网、核糖体丰富 ;神经毡中突触增多 ,突触内可见大量清亮突触小泡。结论 D gal能引起小鼠海马超微结构异常 ;APP17p对此有防治作用
Objective To investigate the effect of APP17p on the ultrastructure of hippocampus of D-galactose-induced aging model mice. Methods The mouse model of aging was established by D gal, and APP17p was injected subcutaneously. After 8 weeks, the hippocampal CA1 region of the mouse was taken for electron microscopic observation. Results ① The axonal microtubule structure of hippocampal neurons in Dgal group was obviously dissolved. The number of mitochondria was more than that of APP17p + D group, but the difference was not significant. The mitochondria were swollen with vacuolation and electron density increase. The number of endoplasmic reticulum was significantly reduced; the synapse in neuropil was significantly reduced compared with that in APP17p + D group (P <0.05); no abnormal changes were found in the structure of hippocampal neurons. (2) The microtubules of hippocampal neurons in AP17p + D group were normal and the microfilaments were clear; the mitochondrial membrane and structure were normal, the mitochondrial ridges were clear; the rough endoplasmic reticulum and ribosomes were abundant; the number of synapses in neuropil was increased; Within a large number of clear synaptic vesicles. Conclusion D gal can induce ultrastructural abnormalities in the hippocampus of mice. APP17p can prevent and treat this disease