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目的:不同种源玉竹多糖含量比较及分析,为玉竹种质资源利用与良种选育提供依据。方法:收集全国主分布区玉竹野生种质资源21份,考察农艺性状、经纬度与海拔,用苯酚-硫酸法测定多糖含量,用Excel,SPSS,DPS软件进行数据分析。结果:供试玉竹种源间农艺性状与多糖含量存在极显著差异(P<0.01),湖南与四川种源多糖含量较高,其次为浙江种源;多糖含量与叶宽、根状茎直径、海拔相关性较低,在叶大而狭长,茎较粗,果实较大的低经纬度种源中较高;12个性状可简化为5个主成分,累计贡献率达85.44%;21个种源可划分为4个类群,类群Ⅰ植株较高大,茎较粗,果实大,叶片较多而狭长或宽厚,根状茎产量高,综合性状表现较好。结论:共筛选出8个优良种源,良种选育时,可优先在湖南与四川平昌种源中选择,其次为浙江临安、浙江磐安与吉林抚松种源。
OBJECTIVE: To compare and analyze the polysaccharides content of Polygonatum from different provenances, and to provide the basis for utilization of Polygonatum germplasm resources and breeding of elite varieties. Methods: Twenty-one wild germplasm resources of Polygonatum odoratum were collected from the main distribution areas in China. The agronomic characters, latitude, longitude and altitude were investigated. The content of polysaccharides was determined by phenol-sulfuric acid method. The data were analyzed by Excel, SPSS and DPS software. Results: There was significant difference (P <0.01) between agronomic traits and polysaccharides content in provenances of Yuyu bamboo, higher content of polysaccharides in provenances of Hunan and Sichuan, followed by provenances in Zhejiang, polysaccharides content and leaf width, diameter of rhizome , Lower in altitude, higher in long and narrow leaves, thicker stems and higher fruit trees with low latitude and longitude; 12 traits could be simplified into 5 main components with a cumulative contribution rate of 85.44%; 21 species The source can be divided into four groups. The plants of group Ⅰ are tall, thick stems, large fruit, more leaves, narrow or thick, high rhizome yield and good comprehensive characters. CONCLUSION: Eight elite provenances were selected. Breeding of elite seeds could be preferentially selected from the provenances of Pingchang, Hunan Province and Pingchuan of Sichuan Province, followed by Lin’an of Zhejiang Province, Pan’an of Zhejiang Province and Fusong of Jilin Province.