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目的：探讨人参茎叶皂甙对Ｄ－半乳糖拟衰老大鼠的作用。方法：Ｗｉｓｔａｒ雌性大鼠自５０日龄起每日皮下注射Ｄ－半乳糖４８ｍｇ／ｋｇ，连续６周。其中一组同时喂饲人参茎叶皂甙（２５ｍｇ／２０ｇ饲料比例掺入）。应用邻苯三酚法、ＴＢＡ比色法、放射免疫分析法分别测定下丘脑超氧化物歧化酶（ＳＯＤ）活性、脑组织丙二醛（ＭＤＡ）含量、血清及原代垂体细胞培养液中催乳素（ＰＲＬ）浓度。结果：人参茎叶皂甙可阻止Ｄ－半乳糖诱发下丘脑ＳＯＤ活性下降、脑组织ＭＤＡ含量增加、血清ＰＲＬ升高以及垂体细胞对促甲状腺素释放激素（ＴＲＨ）、血管活性肠肽（ＶＩＰ）及多巴胺（ＤＡ）刺激的反应性降低等作用。结论：人参茎叶皂甙有对抗Ｄ－半乳糖致“衰老”的作用
Objective: To investigate the effect of ginseng stem and leaf saponin on D-galactose-like aging rats. METHODS: Wistar female rats were injected subcutaneously with D-galactose 48 mg/kg daily for 60 weeks from the age of 50 days. One group was fed with Ginseng stem and leaf saponin at the same time (25 mg/20 g feed ratio was incorporated). The activities of superoxide dismutase (SOD) in the hypothalamus, malondialdehyde (MDA) content in brain tissue, and serum and primary pituitary cells in culture medium were measured by pyrogallol method, TBA colorimetric method, and radioimmunoassay. (PRL) concentration. RESULTS: Ginseng stem and leaf saponins could prevent D-galactose-induced hypothalamic SOD activity decrease, brain tissue MDA content increase, serum PRL elevation, and pituitary cells on thyrotropin releasing hormone (TRH), vasoactive intestinal peptide (VIP) and Dopamine (DA) stimulates the decrease of reactivity and other effects. Conclusion: Ginseng stem and leaf saponins have anti-D-galactose-induced aging